Case Presentation - Spring, 2014
Written by: Maria Lopez, student, Cleveland Clinic School of Cytotechnology, Cleveland, Ohio
Patient Age and History: Female, age 46
Specimen type: FNA-Left Breast, ThinPrep® Non-Gyn cytology
Patient History: Status post bilateral mastectomies for Grade 2 Infiltrating Ductal Carcinoma approximately 1 year prior. Now with mass at mastectomy site on left
Cytologic diagnosis: Negative, consistent with fat necrosis
Case provided by: Milstead Pathology, Conyers, Georgia
Reminder: You may click on any slide image for an enlarged view.Etiology:
Fat necrosis of the breast is a phenomenon that occurs within breast adipose tissue following exposure to trauma. A wide range of occurrences can cause trauma but physical injuries, such as accidental falls and damage relating to surgical procedures such as lumpectomy, are the most common. Other causes include implant removal, breast reconstruction, biopsy, mammary duct ectasia, breast infection, and exposure to radiotherapy. As a result of the trauma, the adipocytes undergo necrosis and a benign inflammatory process results from the saponification of fat by blood and tissue lipase.1 Microcalcification and lipid cyst formation can occur but cystic degeneration can leave behind an oil filled cavity. The calcifications that develop within the cyst walls are surrounded by fibrosis, and these can be detected by mammography.
Both females and males can be diagnosed with fat necrosis of the breast. Symptoms may or may not manifest; however, when present the clinical features can vary. Some patients may have a single, smooth rounded lump while others may possess multiple lumps. Irregular masses with overlying skin retraction may also be present. Other features associated with underlying fat necrosis include pain, skin thickening or retraction, nipple retraction, erythema, ecchymosis, and lymphadenopathy.1,6 Furthermore, mammographic features of fat necrosis also vary depending on the amount of fibrosis present. Minimal fibrosis is associated with earlier stages, where the lesion is seen as an oil cyst or a radiolucent mass. Calcifications will develop in the progression of fat necrosis and these can take on either a benign, ring-like structure or rod-like, irregular shapes suspicious for malignancy.
Treatment and Prognosis:
Because fat necrosis of the breast presents in various ways, management does not follow strict guidelines. Rather, it is contingent upon the presenting symptoms and the combined assessment of imaging findings and FNA biopsy. Fat necrosis should resolve itself, so the lesions are generally not removed immediately. If the mass does not decrease in size or if it grows, surgical excision occurs to rule out a malignancy. If pain presents, pain medication can also be prescribed at various levels (narcotics or non-narcotic analgesics).
On FNA biopsy it is possible to sense a grainy texture through the penetrating needle. Greasy material will be collected and may bead up on air-dried slides. Smears of fat necrosis are generally hypocellular. There will be varying amounts of infiltrating histiocytes, hemorrhage, fibrosis, and calcification visualized based on the progression of fat necrosis. The typical cytological presentation includes histiocytes with foamy, vacuolated cytoplasm, multinucleated giant cells, blood pigment, and possibly foreign body granulomas. Neutrophils, lymphocytes, and plasma cells can be seen in the background with acute inflammation predominating in the earlier stages. Chronic inflammation will predominate in the later stages and it, along with the formation of granulation tissue and fibrosis, can result in the creation of scar tissue which can mimic cancer. The few epithelial cells present may be considered atypical because of their prominent nucleoli, nuclear pleomorphism, hyperchromasia, and mitotic figures.3
The two main tumors that must be distinguished from chordoma are chondrosarcoma and metastatic adenocarcinoma. In addition, myxopapillary ependymoma must also be ruled out. Both chordoma and chondrosarcoma will shed cells that are finely vacuolated, however, cells with large, multiple vacuoles are lacking in a chondrosarcoma. A key difference between myxopapillary ependymoma and chordoma is the presence of ciliated cells in myxopapillary ependymoma. Immunohistochemical staining can help in the differentiation of these lesions as well. Both chordoma and chondrosarcoma stain positive for S100, while only chordoma will be positive with EMA and panCK. A further distinction is that chondrosarcoma will be positive for D2-40. To distinguish between chordoma and myxopapillary ependymoma EMA and GFAP are the stains of choice. Myxopapillary ependymoma will stain negative for EMA but positive for GFAP. Chordoma will have the opposite staining results - positive for EMA and negative for GFAP. Because chordomas stain positive for some epithelial markers, metastatic adenocarcinoma must be ruled out. Combining the midline location of the tumor with cytological criteria such as physaliphorous cells that contain large, multiple vacuoles in the cytoplasm will lead to a diagnosis of chordoma.
- Atkinson, B., Silverman, J. Atlas of Difficult Diagnoses in Cytopathology. 1998: 290, 485-6, 504.
- Atkinson, B., Silverman, J. Atlas of Difficult Diagnoses in Cytopathology, Second Edition. 2004: 182, 642, 659.
- Cibas, E., Ducatman, B. Cytology Diagnostic Principles and Clinical Correlates Second Edition. 2003: 435.
- McKee, G. Cytopathology. 1997: 351-3.
- Bibbo, M. Comprehensive Cytopathology, Second Edition. 1997: 534.
- Cho, HY., Lee, M., Takei, H., Dancer, J., Ro, JY., Zhai, QJ., Immunohistochemical comparison of chordoma with chondrosarcoma, myxopapillary ependymoma, and chordoid meningioma. Applied Immunohistochemistry and Molecular Morphology. 2009, March; 17(2): 131-8.
- Koss, M.D., Woyke, S., Olszewski, W., Aspiration Biopsy, Cytologic Interpretation and Histologic Bases, Second Edition. 1992: 645-6