Molecular Methods Quiz

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1. The technique of removing DNA or RNA from surrounding cellular material is known as:
 amplification
 purification/extraction
 sequencing
 none of the above

2. What method elucidates the nucleic acid sequences of short segments without electrophoresis?
 PCR
 FISH
 Pyrosequencing
 Restriction Fragment Length Polymorphism (RFLP)

3. There are four types of probes that are typically used for in situ hybridization.
 True
 False

4. What is the molecular cytogenetic technique that is used to detect chromosomal abnormalities in cells or tissue?
 FISH
 Southern blot
 PCR
 RFLP

5. Invader® technology and Hybrid Capture are both examples of target amplification technologies.
 True
 False

6. A method that probes for the presence of a specific DNA target within a purified and non-amplified DNA sample is called:
 Electrophoresis
 Pyrosequencing
 Southern blot
 FISH

7. A single stranded DNA molecule is primed and enzymatically extended to incorporate new deoxynucleotides (dNTPs) and dideoxy-nucleotides (ddNTPs). This procedure is called:
 Chain terminator (Sanger Method)
 Chain release (Smith Method)
 Chain reaction (PCR Method)
 Chain sequence (Long Method)

8. What technique for separation of DNA, RNA or protein molecules uses an electric current applied to a gel matrix?
 PCR
 FISH
 Pyrosequencing
 Gel Electrophoresis

9. Strand displacement amplification and Transcription-based amplification are both isothermal nucleic acid amplification techniques.
 True
 False

10. The Invader® reaction requires which of the following to take place?
 Invader/primary hybridization to target
 Overlap of the Invader and primary probes to create a specific structure
 Recognition and cleavage by Cleavase® enzyme
 All of the above


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